JIS Z 2801:2010 Antibacterial Products-Test for Antibacterial Activity and Efficacy

The Japanese Industrial Standard (JIS) Z 2801 test method is designed to evaluate the antibacterial activity of a variety of surfaces including plastics, metals and ceramics.  Two types of bacteria are used to challenge the test surfaces: Staphylococcus aureus and Escherichia coli.  Each test specimen (50 mm x 50 mm) is placed in a petri dish and the test inoculum is added onto the specimen.  A film is then added to cover the entire test specimen.  Triplicate specimens are inoculated for each data point.  Immediately after inoculation, untreated specimens are processed to count viable organisms at Time 0.  Untreated and treated specimens are then incubated at 35oC for 24 h.  Test organisms are enumerated by washing specimens in a neutralizing broth and plating using serial dilutions.

The JIS Z 2801 test method is one of the most commonly requested tests for determining the antibacterial activity on surfaces.  It is also almost identical to ISO 22196: Measurement of antibacterial activity on plastics and other non-porous surfaces.

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